Genetic Variations in a Conserved 5'-Untranslated region of Hepatitis C Virus Isolated From Egypt

ملخص البحث

Random out patients from the center of cardiac and digestive system, Sohag, Egypt, aged from 20 to 55 years old, negative for HBs antigens and HBs antibodies were screened for anti-HCV antibodies. Ninety-two positive samples were confirmed by RT-PCR for HCV. HCV-infected patients were received 12 vials of pegylated IFN alpha-2a (180 mcg/0.5 ml; fixed dose/week- Roche) plus ribavirin (1000 mg for ≤ 75 Kg or 1200 mg for > 75Kg) and were followed up by RT-PCR. The results showed that 67 patients (72.8%) were responded to the treatment (group A), while 25 patients (27.2%) were non responding (group B). The two groups were screened for Serum alanine transaminase (ALT), serum aspartate transaminase (AST), serum alpha fetoprotein (AFP), serum Albumin, serum creatinine, serum TSH, Hemoglobin (Hb), White blood cells count (WBCs) and Platelets count. Hematological disorders and ALT elevation revealed common side effects of the treatment. The side effects were higher within group B than that of group A. Three random HCV-RNA corresponds to group B were studied for sequence variations. Sequencing of 223 nucleotide of the 5'-untranslated region (5′-UTR) was performed. Nucleotide sequence identity was 96.9-98.2 %. Seven nucleotide variations were found at positions 74, 92, 112, 113, 133, 172, and 180. One nucleotide insertion was detected in Sohag-Maragha isolate at position 212. Neighbourjoining (NJ) tree revealed that the three HCV isolates classified as genotype-4. Sohag-Monshah isolate was more closely related to Gabon isolate than to the Egyptian isolates. Sohag-Monshah isolate could be a new subtype but this will require an in-depth exploration on sequence variability in at least two coding regions

مكان النشر:

International J. Virol.، العدد 7 (3): 91 -99.

تاريخ البحث:

2011

الكلمات الدلالية:

HCV, RT-PCR, IFN, 5′-UTR

ملفات: