EVALUATION OF PROTECTIVE EFFECT OF WHEAT GERM ON CHLORPYRIFOS INDUCED TOXICITY IN RATS.

ملخص البحث

Chlorpyrifos (CPF) is a widely used insecticide causing harmful effects in human beings and animals. Determining effective and economic antioxidants is an ultimate goal for all toxicologists. In this regard, the current study focused on the protective effect of wheat germ (WG) administered as 20% of diet for 45 days against the toxic effects of CPF given at 50 mg/kg/day for the last 3 days of experiment by gastric intubation. CPF induced hematological changes in the form of reduction of red cell count (RBC), hemoglobin (Hb) level and packed cell volume (PCV) with leukocytosis, neutrophilia and lymphocytopenia. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) activities and serum concentrations of iron, total protein, globulin, ceruloplasmin and haptoglobulin were elevated. Malondialdehyde (MDA) level increased with concomitant reduction of reduced glutathione (GSH) level and catalase (CAT) activity in liver, brain and spleen tissues. This was associated with histopathological alterations in these organs. Moreover, CPF produced genotoxic effects in liver and brain as demonstrated by comet assay. Administration of WG-enriched diet ameliorated most of the toxic effects produced by CPF. In conclusion, it is recommended to use WG to protect animals and human being from harmful effects of CPF and other organophosphates

مكان النشر:

Int. J. Adv. Res.، العدد 5(12), 991-1002.

تاريخ البحث:

2017

الكلمات الدلالية:

Chlorpyrifos, Wheat Germ, Hematology, Antioxidants, Comet, Histopathology

ملفات: